Composite
Part:BBa_K2757000:Design
Designed by: Bhuvana Sudarshan Group: iGEM18_Oxford (2018-10-10)
Art-175 fused with N-terminal DsbA 2-19 signal peptide and unidirectional pTet
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 477
Illegal AgeI site found at 676 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
Art-175 is synthesised by fusion of the SMAP-29 sheep myeloid peptide with the KZ144 endolysin originating from Pseudomonas aeruginosa. pTet promoter sequence taken from BBa_R0040.