Composite

Part:BBa_K2757000:Design

Designed by: Bhuvana Sudarshan   Group: iGEM18_Oxford   (2018-10-10)


Art-175 fused with N-terminal DsbA 2-19 signal peptide and unidirectional pTet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 477
    Illegal AgeI site found at 676
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Art-175 is synthesised by fusion of the SMAP-29 sheep myeloid peptide with the KZ144 endolysin originating from Pseudomonas aeruginosa. pTet promoter sequence taken from BBa_R0040.

References